The effect of tourniquet compression on axonal transport in sciatic nerve of rats
10.3760/cma.j.issn.0254-1416.2011.03.006
- VernacularTitle:止血带压迫对大鼠坐骨神经轴浆运输的影响
- Author:
Lei WANG
;
Ran XIAO
;
Ye WANG
;
Xiaowen LIU
;
Xiaoming DENG
- Publication Type:Journal Article
- Keywords:
Tourniquets;
Axonal transport;
Sciatic nerve
- From:Chinese Journal of Anesthesiology
2011;31(3):289-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tourniquet compression on axonal transport in sciatic nerve of rats.Methods Twenty-four 12-week old male SD rats weighing 250-300 g were randomly divided into 4groups according to the duration of tourniquet compression(n=6 each):1,2,4 and 12 h.The tourniquet was applied to the middle 1/3 of thigh.In each animal whether the left or right thigh was compressed was determined by a flip of coin.The tourniquet was released for 10 min after every hour of compression.A 3-cm segment of sciatic nerve was removed at the end of tourniquet compression(1.5 cm proximal and 1.5 cm distal to the site of compression).Immuno-histochemistry was used to measure the expression of insulin-like growth factor-1(IGF-1)in the sciatic nerve.The ratio of average optic density of the compressed sciatic nerve to that of control was used to estimate the degree of IGF-1 accumulation.The regression equation of the interaction between the duration of compression and accumulation of IGF-1 was analyzed.Results There was significant accumulation of IGF-1 in the sciatic nerve proximal to the compressed site.The accumulation increased with the duration of compression.There was no significant accumulation of IGF-1 in the sciatic nerve distal to the compressed site.The regression equation of the interaction between the duration of compression(X)and accumulation of IGF-1(Y)was Y=0.422X+0.887.Conclusion Tourniquet compression of sciatic nerve can inhibit axonal transport.The accumulation increases with the duration of compression.
