Anti-tumor effects induced by gene vaccines co-expressing truncated human prostate specific membrane antigen gene and mouse 4-1BBL
10.3760/cma.j.issn.1000-6702.2011.06.015
- VernacularTitle:前列腺特异性膜抗原和4-1BB配体共表达基因疫苗诱导的抗肿瘤作用
- Author:
Youlin KUANG
;
Xiaodong WENG
;
Xiuheng LIU
;
Zhiyuan CHEN
;
Hengcheng ZHU
;
Hui CHEN
;
Botao JIANG
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
4-1BBL;
Vaccine;
Animals,laboratory
- From:
Chinese Journal of Urology
2011;32(6):403-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of m4-1BBL on the anti-tumor effects induced by truncated human prostate specific membrane antigen (tPSMA) gene in mice. Methods A eukaryotic expression plasmid encoding tPSMA and m4-1BBL (pDC316-tPSMA-IRES-m4-1BBL), pDC316-tPSMA and pDC316 were constructed. C57BL/6 mice were vaccinated in the quadriceps femoris, respectively. The CTL activity of spleen cells from the immunized mice against prostate cancer RM-1-tPSMA was detected by CCK-8 kit in vitro. The tumor growth was then observed. Results The target cell specific cytotoxicity rate induced by pDC316-tPSMA-IRES-m4-1BBL was 42.6%, compared to 24.8% in the pDC316-tPSMA group and 10.8% in the pDC316 group. The difference was significant (P<0.05). The volume of tumor in the pDC316 group was 2657.4mm3 7 d after vaccination, compared to 1334.5 mm3 in the pDC316-tPSMA group, 9 d after vaccination. In the pDC316-tPSMA-IRES-m4-1BBL group, the tumor volume was 445.8 mm3, 12d after vaccination. The difference was significant (P<0.05). Conclusion Gene vaccines co-expressing tPSMA gene and m4-1BBL gene could significantly enhance anti-prostate cancer effects in mice.
