Expression of FSH1 in Microsporum canis before and after the induction by skin tissue from the scalp and foreskin of children
10.3760/cma.j.issn.0412-4030.2011.06.020
- VernacularTitle:儿童头皮与包皮组织诱导前后犬小孢子菌FSH1的表达
- Author:
Yiping ZHU
;
Zhenying ZHANG
;
Juan PANG
;
Guoling YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Dermatology
2011;44(6):441-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the significance of FSH1 in the pathogenicity of M. canis. Methods Thirty M. cam's strains from tinea capitis lesions and 30 M. canis strains from tinea corporis lesions were cultured, passaged, and induced by medium containing skin tissue of scalp or foreskin from children. Semi-quantitative reverse transcription (RT)-PCR was carried out to detect the expression of FSH1 mRNA in the firstand fifth-generation M. canis strains, as well as M. canis strains induced by the skin tissues. Results The mRNA expression of FSH1 was higher in M. canis strains derived from tinea capitis lesions than in those from tinea corporis lesions (P < 0.01), but there was no significant difference between the first-generation and fifthgeneration M. canis strains (P> 0.05). The skin tissue from scalp and foreskin induced a significant elevation in the mRNA expression of FSH1 in these M. canis strains (F = 2025.713, 1833.139, both P< 0.01), and the inductive effect of the scalp tissue was different from that of the foreskin tissue (P < 0.01). Conclusions The FSH1 mRNA expression is different in M. canis isolated from different body sites. Local skin tissue has an inductive effect on the expression of FSH1 mRNA, and the inductive effect of scalp tissue is more apparent than that of foreskin tissue.
