Identification of IMP-1 Metallo-β-lactamase in carbapenem-resistant Klebsiella pneumoniae
10.3760/cma.j.issn.1674-2397.2011.03.006
- VernacularTitle:耐碳青霉烯类抗生素肺炎克雷伯菌IMP-1型金属β-内酰胺酶的检测
- Author:
Jing LI
;
Zhidong HU
- Publication Type:Journal Article
- Keywords:
Carbapenem;
Klebsiella pneumoniae;
Integron;
Drug resistance
- From:
Chinese Journal of Clinical Infectious Diseases
2011;4(3):149-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genes of a carbapenem-resistant Klebsiella pneumoniae strain isolated from a patient. Methods The antibiotic sensitivity test of a multidrug-resistant Klebsiella pneumoniae strain was done according to K-B and MIC method. Metallo-β-lactamase was detected by Modified Hodge Test and EDTA-disk synergy test. Both nine genes encoding β-lactamases, including blaKPC, blaIMP , blaVIM , blaSME , blaCTX-M , blaSHV, blaDHA , blaACT, Class Ⅰ integrase and Class Ⅰ integron were detected by PCR. Positive products were sequenced. Results The Klebsiella pneumoniae was resistant to carbapenems, cephalosporins, cefoxitin, ampicillin and trimethoprim/sulfamethoxazole. Only susceptible to aztreonam, gentamicin, tobramycin, amikacin, ciprofloxacin and levofloxacin. The blaIMP-1 and Class Ⅰ integron were positive. The blaIMP gene was identified by PCR and DNA sequencing confirmed that the gene belong to IMP-1 type Metallo-β-lactamase gene. The strain also carried Class Ⅰ integron and IMP-1 was located in Class Ⅰ integron 5'. Conclusions It is the first detection of IMP-1 Metallo-β-lactamase in Klebsiella pneumoniae. The production of IMP-1 carbapenemase is the main mechanism of carbapenem-resistant in Klebsiella pneumoniae, and multidrug resistance is related to ClassⅠ integron.
