The study of changes in expression of oxidative stress-related genes in steriod-induced necrosis of femoral head
10.3760/cma.j.issn.0253-2352.2011.07.013
- VernacularTitle:大鼠激素性股骨头坏死组织氧化应激相关基因的差异表达
- Author:
Peijian TONG
;
Qiang MAO
;
Chengliang WU
;
Hongting JIN
;
Luwei XIA
- Publication Type:Journal Article
- Keywords:
Rats,Wistar;
Femur head necrosis;
Gene expression;
Prednisolone
- From:
Chinese Journal of Orthopaedics
2011;31(7):794-799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify significantly differentially expression of rat by genes chip,try to find out the initiating factors and molecular mechanisms that oxidative stress originate or strengthen the steriod-induced necrosis of femoral head(SINFH).Methods Twenty Wistar rats were dived into experimental group and control group randomly.The rats were injected intraperitoneally whith endotoxin,and then injected intramuscularly with high-dose methylprednisolone or saline in experimental group and control group respectively.The mRNA was extracted from the femoral head of rats inevery group,and the cDNA probes were obtained by inverse transcript,and then carried out microarray detection.The quantitative RT-PCR was used to confirm the results of the microarray.Results Histopathological findings revealed that the experimental group rats had femoral head necrosis,trabecular bone disorders,thinning,bone cell necrosis,and the rate of empty lacunae increased,and in control group no femoral head osteonecrosis was found.Total of 27 differentially expressed genes were found,and of which 4 genes(COX6A2,COX4I2,SOD3,and DUSP1)were significantly different.The expression of these 4 genes were down-regulated.The functions of these four genes involved in inhibition of reactive oxygen species(ROS)generation,accelerated removal of ROS and protection tissue from oxidative damage and so on.Conclusion The expression of oxidative stress-related genes in SINFH of rats exist change.COX6A2,COX4I2,SOD3,and DUSP1 are key genes in process of oxidative stress originate or strengthen the SINFH.
