Study on the anti-angiogenic activity of tumstatin related peptide T3 mediated by short peptide to osteosarcoma vascular
10.3760/cma.j.issn.0253-2352.2011.06.025
- VernacularTitle:靶向短肽介导的肿瘤抑素活性T3片段对骨肉瘤的疗效
- Author:
Zandong ZHAO
;
Zhanjun SHI
;
Lanbo YANG
;
Mingqiang GUAN
;
Peng LI
;
Jun XIAO
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
Tumor suppressor proteins;
Vascular endothelial growth factors
- From:
Chinese Journal of Orthopaedics
2011;31(6):699-705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory effect of tumstatin related peptide T3 mediated by short peptide to osteosarcoma vascular. Methods Through MTS assay, wound healing assay, the inhibitory effect of targeting-T3 peptide and T3 peptide on the human umbilical veil endothelial cell was studied in vitro. After the preparation of 50 nude mice model bearing osteosarcoma, the nude mice bearing too large or too small tumors were eliminated and the left ones were divided into 4 groups (6 animals for each group: T3 peptide, targeting-T3 peptide, CTX, PBS) randomly. Through weight of tumor, histopathologicol slice and immunohistochemical methods. The inhibitory action of targeting-T3 peptide and T3 peptide on the neoge-netic vascular of osteosarcoma implanted in nude mouse was studied. Results In vitro, both T3 peptide and targeting-T3 peptide effectively inhibited the proliferation of human umbilical veil endothelial cell. In the experiment of vivo, the average weight of tumor of targeting-T3 peptide group was (1.104?.247) g, the average weight of the T3 peptide group was (1.484?.369) g. There was the statistical difference in tumor inhibition on the osteosarcoma betweent the targeting-T3 group and T3 group (F=16.353, P=0.000). The positive rate of vascular endothelial growth factor and metastasis in the lung in the targeting-T3 peptide group all descended than the T3 peptide group. Conclusion Because of the short peptide to osteosarcoma vascular, targeting-T3 peptide could significantly restrain the development of osteosarcoma. Coupling short peptide to T3 peptide increase the selective binding of T3 peptide to osteosarcoma vascular.
