Research of interference Livin gene inhibits growth of QBC939 cell
10.3760/cma.j.issn.1007-8118.2011.07.018
- VernacularTitle:干扰Livin基因对人胆管癌QBC939细胞增殖影响的研究
- Author:
Shengxiong CHEN
;
Ping CUI
;
Chen LI
;
Ming HONG
;
Shaoyou LI
;
Lele DUAN
- Publication Type:Journal Article
- Keywords:
Livin;
Gene interference;
Bile duct cancer;
Cell proliferation
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(7):580-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of transfection of livin antisense oligodeoxynucleotide (Livin ASODN) on Livin mRNA and Livin protein expression and proliferation of QBC939 cells.Methods Livin ASODN was transfected into cell line QBC939 by LipofectamineTM 2000. Fluorescence microscopy was used to observe the ASODN transfected cells and to calculate the rate of transfection.to measure Livin mRNA and Livin protein expression by RT-PCR and immunohistochemistry and con-focal laser scanning microscopy after the transfection. Changes in cell proliferation were detected by MTT. Results The highest efficiency was at 24 hours after 500 nmol/L Livin ASODN transfection.The results of MTT showed that the inhibition of cell proliferation of QBC939 cells was most obvious at 60 hours after Livin ASODN transfection (P<0. 05). The level of Livin mRNA and Livin protein expression in the ASODN group was obviously lower than that in the control group (P<0. 05).Conclusion The transfection of Livin ASODN inhibited Livin gene and Livin protein expression, and obviously inhibited the proliferation and depressed the vitality of QBC939 cells.
