Effects of X-rays on CC-chemokine receptor 7 expression in human lung cancer A549 cells
10.3760/cma.j.issn.0254-5098.2011.03.013
- VernacularTitle:X射线对人肺癌A549细胞CCR7表达影响的研究
- Author:
Cuilan WANG
;
Qisheng JIANG
;
Yue ZOU
;
Fengsheng LI
;
Wei LI
;
Xiujun SONG
;
Rui HE
;
Lu WANG
- Publication Type:Journal Article
- Keywords:
Lung cancer;
CC-chemokine receptor 7 (CCR7);
X-rays
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(3):299-302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of X-ray radiation on CC-chemokine receptor 7(CCR7) expression in human non-small cell lung cancer (NSCLC) cells.Methods Humanadenocarcinoma cells of the line A549 were cultured and irradiated by X-ray at the absorbed doses of 2,4,6,and 8 Gy respectively by linear accelerator (with the source skin distance of 100 cm and dose rate of 442.89 cGy/min).The relative levels of CCR7 mRNA and protein expression in the A549 cells were respectively detected by real time-PCR and Western blotting 4,12,24,48,and 72 h after radiation.Untreated A549 cells were used as control group.Results The expression levels of CCR7 mRNA and protein in the A549 cells began to increase since 4 h after radiation and then decreased gradually after they reached the peak.The CCR7 mRNA expression levels 72 h after radiation of the 6 and 8 Gy groups were still significantly higher than those of the control group (t = 6.75-7.26,both P < 0.01),and the CCR7 protein expression levels of the 2 and 6 Gy group were still significantly higher than those of the control group(t=11.13-14.17,both P <0.01).Then the CCR7 protein expression levels of the 4 and 8 Gy groups decreased to the control group level 48 and 72 h after radiation respectively.Conclusions The CCR7 mRNA and protein expression levels in the NSCLC cells increase after X-ray irradiation,which may be correlated with the promotion of proliferation and metastasis of NSCLC cells by X-ray irradiation at a certain dose.
