Dose-effect relationship and time-effect relationship of T cell receptor gene mutation induced by γ-rays in human lymphocytes of peripheral blood
10.3760/cma.j.issn.0254-5098.2011.03.010
- VernacularTitle:γ射线诱发人外周血淋巴细胞T细胞受体基因突变剂量-效应和时间-效应关系
- Author:
Xuesong QI
;
Huimin Lü
;
Chunyan WANG
;
Wei ZHANG
;
Shuxia HAO
;
Qiao GOU
;
Peng TONG
;
Qingjie LIU
;
Xu SU
- Publication Type:Journal Article
- Keywords:
γ-rays;
T cell receptor (TCR);
Dose-effect relationship;
Time-effect relationship;
Biological dosimeter
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(3):286-289
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the dose-effect relationship and time-effect relationship of T cell receptor (TCR) gene mutation induced by γ-rays in lymphocytes of human peripheral blood.Methods Samples of peripheral blood were collected from 10 healthy adults and lymphocytes were separated.Four samples from males used to fit time-effect curve were exposed to γ-rays at the doses of 0,0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,and 5.0 Gy,respectively,and 6 samples from 3 males and 3 females used to fit dose-effect curves were exposed to γ-rays of the dose of 2 Gy.Flow cytometry was used to detect the mutation frequency of TCR gene (TCR MF).Radiation dose-effect curves and time-effect curves were fitted and optimal mathematical models were selected respectively.Results The optimal mathematical model for radiation dose-effect was quadratic equation model:TCR MF = 92.14 + 22.61D2 (R2adj = 0.65).The optimal mathematical model for radiation time-effect was quadratic polynomial equation model:TCR MF = 3.74 + 743.66T + 308.64T2 (R2adj = 0.79).Conclusions TCR MF is increased as the γ-rayirradiation dose increases within the range of 0-5 Gy,and TCR MF is increased with the lapse of time within the range of 4 days after γ-ray radiation.
