Screening radiosensitizing-related genes mediated by element in lung adenocarcinoma A549 cells by using gene chip
10.3760/cma.j.issn.0254-5098.2011.03.005
- VernacularTitle:基因芯片筛选榄香烯乳增强肺腺癌A549细胞放射敏感性相关基因
- Author:
Zhuo ZHANG
;
Lijuan ZOU
;
Yingying TIAN
- Publication Type:Journal Article
- Keywords:
Gene chip;
Elemene;
Radioensitizing;
Lung adenocareinoma A549 cells
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen radiosensitizing-related genes mediated by elemene in lung adenocarcinoma A549 cells by using gene chip. Methods MTT test was used to calculate the IC50 of elemene. ①The effect of radiosensitivity was detected by colony forming assay. A549 cells were divided into 2 groups: radiation group and radiation + elemene group. Oligonucleotide chip was used to screen the gene expression changes of A549 cells from these 2 groups. The up-regulated gene Egr-1 and the down-regulated gene CyclinDl were selected to undergo RT-PCR so as to confirm the reliability of the result. Results MTT test showed the elemene inhibited the proliferation of the A549 cells dose-dependently. The IC50 value of elemene on the A549 cells was 120 mg/L. ②10 mg/L elemene had radiosensiting effect on A549 cells.The values of SERDO and SERDq obtained from the survival curve were (1.54±0. 20) and (1.43±0. 15 )respectively. Gene chip screened 122 differentially-expressed genes, including 89 up-regulated genes and33 down-regulated genes. ③These altered genes could be related to cell structure, substance metabolism,cell proliferation, cell differentiation, signal transduction, material transport, DNA repair, apoptosis,immune response and so forth. The RT-PCR results of Egr-1 and Cyclin D1 were consistent with the genenchip analysis.Conclusions The mechanism of elemene enhancing the radiosensitivity of lung adenoearcinoma A549 cells is the result of participation and collaboration of multiple genes. Further study of the newly-discovered differentially-expressed gene helps find out new radiosensitizational targets of elemene.
