Macrophage-inducible C-type lectin wets selectively expressed on macrophages and dendritic cells in synovial fluid from patients with rheumatoid arhritis
10.3760/cma.j.issn.1007-7480.2011.07.002
- VernacularTitle:凝集素样受体巨噬细胞诱导性C型凝集素样受体在类风湿关节炎患者巨噬/树突状细胞中表达及其临床意义
- Author:
Jianping GUO
;
Xiaolin SUN
;
Yingni LI
;
Xinyu WU
;
Jing HE
;
Ru LI
;
Yuan AN
;
Jing ZHAO
;
Sisi PAN
;
Chun LI
;
Zhanguo LI
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Macrophages;
Dendritic cells;
Macrophage-inducible C-type lectin
- From:
Chinese Journal of Rheumatology
2011;15(7):435-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression pattern of macrophage-inducible c-type lectin (MINCLE)on Macrophage(Mφ),myeloid dendritic cell (mDC)and plasmacytoid DC(pDC)in peripheral blood (PB)and synovial fluid(SF)in patients with rheumatoid arthritis (RA).Methods For mRNA expression of MINCLE,253 RA patients and 71 healthy control subjects were enrolled.The mRNA level of MINCLE was determined by real-time PCR.For protein expression of MINCLE,18 patients with RA,5 patients with osteoarthritis(OA)and 12 healthy control subjects were enrolled.The expression of MINCLE on Mφ,mDC and pDC were detected by flow cytometry.The differences of MINCLE expressions in PB between RA patients,OA patients and healthy controls,or differences between PB and SF in RA patients were analyzed using Mann-Whitney U test or paired-samples t test.Results ①Compared to the healthy controls,RA patients showed elevated mRNA expression level of MINCLE in PBMCs[(1.65±0.36)vs (0.37±0.06),U=6057,P=2.75×10-5].②At protein level,MINCLE was hardly detected in Mφ,mDC and pDC in PB of OA patients and healthy controls.In SF,MINCLE was highiy expressed on mDC in RA patients,compared with that in OA patients[(34.8±4.4)%,U=0,P=2.6×10-3].In RA patients,the expression level of MINCLE was remarkably elevated in Mφ,mDC and pDC in SF compared with that in PB[Mφ(2.01±0.53)%vs(0.273±0.51)%,t=4.879,P=2.23×10-6;mDC(34.8±4.4)%vs(22.7±5.5)%t=2.535.P=0.017].Conclusion MINCLE is selectively expressed on Mφ.mDC and pDC in SF in RA patients.MINCLE may serve as a potential important marker,or even target,for RA and possibly even for inflammation in general.
