Expression of CD226 mRNA in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus
10.3760/cma.j.issn.1007-7480.2011.06.003
- VernacularTitle:系统性红斑狼疮患者外周血单个核细胞CD226基因表达的研究
- Author:
Yan DU
;
Lingxun SHEN
;
Jing CHEN
;
Jianfang ZHU
;
Likai YU
;
You SONG
;
Rong DU
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Gene;
CD226
- From:
Chinese Journal of Rheumatology
2011;15(6):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of CD226 mRNA in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE) and explore the relation between the gene expression and disease activity, and the relation between the gene expression and Gly307Ser polymorphism of CD226 was also examined. Methods CD226 gene was measured with real-time polymerase chain reaction (qRT- PCR) in PBMCs. The expression levels of CD226 gene in PBMCs were compared between 90 SLE patients and 30 healthy individuals. One-way ANOVA and pearson correlation were used for statistical analysis. Results The expression level of CD226 in the PBMCs of SLE patients (6.8±1.1) was significantly decreased compared to healthy individuals (26.5±6.7) (P＜0.01), while there was no association between mRNA level and genotype (P＞0.05). No correlation between ESR, CRP, ANA, SLEDAI scores, C3 and the expression level of CD226 gene was discovered. Conclusion In Hubei Chinese Han population, CD226-Gly307Ser locus is associated with the development of SLE, while T allele does not impact the expression of CD226 gene, thus the role of CD226 gene in autoimmune diseases should be explored in the future.
