Study of quantitative detection of circulating DNA in the plasma of patients with cervical lesion
10.3760/cma.j.issn.0529-567x.2011.07.005
- VernacularTitle:子宫颈疾病患者血浆循环DNA含量的研究
- Author:
Hong WANG
;
Shiyang PAN
;
Jian XU
;
Meijuan ZHANG
;
Dan CHEN
;
Wenying XIA
;
Yachun LU
;
Yan GENG
;
Bai JIN
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
DNA;
Polymerase chain reaction;
Cervical intraepithelial neoplasia
- From:
Chinese Journal of Obstetrics and Gynecology
2011;46(7):501-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective To quantitatively detect circulating DNA levels in the plasma of patients withcervical lesion and to determine the value for diagnosis of cervical lesion and cervical cancer . Methods Preoperative blood samples were collected from 53 cases of low-grade lesions, 49 cases of high-grade lesions, 44 cases of cervical invasive cancer and 70 cases of healthy women. Plasma DNA was extracted by magnetic bead method (BILATEST DNA kit). The quantity of plasma DNA was determined by duplex real-time quantitative PCR. Results Median plasma DNA level of invasive cervical cancer patients was 61. 59 mg/L (32. 06 - 162. 16 mg/L) , which was significantly higher than that of healthy women [16. 35 mg/L(11. 98 -22.71 mg/L), P < 0.01]. Among invasive cervical cancer patients, median plasma DNA level of squamous carcinoma patients was slightly higher than that of adenocarcinoma (50. 43 versus 47. 31 mg/L,P>0. 05). Median plasma DNA level of stage I patients was lower than that of stage Ⅱ- Ⅲ patients (46. 02 versus 71. 35 mg/L, P <0. 05). Conclusion Quantitatively detecting plasma circulating DNA may be with some application prospect in the diagnosis of cervical diseases.
