Identification and adhesion experiment of microbubbles targeted to angiogenesis

VernacularTitle:血管生成靶向微泡的鉴定及黏附实验
Author: Wei WANG ; Guangjian LIU ; Xiaoyan XIE ; Zuofeng XU ; Lida CHEN ; Guangliang HUANG ; Mingde Lü
Publication Type:Journal Article
Keywords: Ultrasonography; Microbubbles; Integrin alphavbeta 3; Endothelial cells
From: Chinese Journal of Ultrasonography 2011;20(7):621-624
CountryChina
Language:Chinese
Abstract: Objective To identify microbubbles targeted (MBt) to alpha(v)beta(3) (αvβ3) via biotin-avidin bridge and evaluate the adhesion to human umbilical vein endothelial cells (HUVECs) in vitro.Methods MBt produced via biotin-avidin bridge were validated using fluorescence in vitro.Adhesion of αvβ3-integrin targeted MBt (MBαvβ3) to HUVECs was tested using the parallel plate flow chamber (PPFC) test.Results Bright green fluorescence was observed on the biotinylated microbubbles(MBB) incubated with fluorescein isothiocyanate labeled streptavidin (FITC-SA) and on MBB-SA incubated with FITC labeled biotin.There was no fluorescence seen on non-targeted control microbubbles,MBB incubated with FITC labeled protein A and MBB-SA incubated with FITC labeled protein A. The adherent rate of MBαvβ3 was significantly higher than MBt with non-specific antibody (MBN) in PPFC test,with 9.9±3.1 of MBαvβ3 and 0.8±0.3 of MBN adhered to HUVECs,respectively(P<0.05).Conclusions Avβ3 targeted microbubbles using biotin-avidin bridging method is highly efficient and reliable for HUVECs.