Targeted contrast-enhanced ultrasound in the assessment of renal cell carcinoma angiogenesis in nude mice

VernacularTitle:靶向超声造影评价裸鼠肾癌新生血管的实验研究
Author: Shuping WEI ; Bin YANG ; Ninghua FU ; Zhijian YANG ; Chunxiao YAO ; Li LEI
Publication Type:Journal Article
Keywords: Ultrasonography; Microbubbles; Kidney neoplasms; Neovascularization,pathologic
From: Chinese Journal of Ultrasonography 2011;20(7):617-620
CountryChina
Language:Chinese
Abstract: Objective To investigate the value of contrast enhanced ultrasound(CEUS) with microbubbles targeted to vascular endothelial growth factor receptor type 2 (VEGFR2) for imaging tumor angiogenesis in murine tumor models.Methods Established human renal cell carcinomas(RCC) subcutaneous xenograft tumor model in nude mice,microbubbles targeted to VEGFR2(MBV) was prepared,control microbubbles(MBC) and MBV were injected respectively in each mouse,the intensity of each microbubble was compared,the expressions of VEGFR2 in tumors were tested by immunohistochemistry.Results CEUS imaging showed the intensity of MBV and MBC was (6.50±1.43)dB,(2.59±0.99)dB,respectively.There was significantly higher intensity when using MBV compared with MBC (P<0.01).The time to wash out was longer in MBV contrast group compared with MBC group.Immunohistochemistry showed VEGFR2 was highly expressed in novel vessel walls.Conclusions Contrast microbubbles targeted to VEGFR2 can specially enhance the images of RCC and has tremendous significance in the assessment of angiogenesis.