Comparison of two real-time quantitative polymerase chain reaction for detecting HCV RNA virus load in plasma
10.3760/cma.j.issn.1000-6680.2011.07.007
- VernacularTitle:两种实时荧光定量聚合酶链反应检测血清HCV RNA的比较
- Author:
Min XU
;
Jingmin NIE
;
Fengyu HU
;
Weiping CAI
;
Xiaoping TANG
;
Haiyan SHI
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Viral load;
Fluorescence real-time quantitative polymerase chain reaction
- From:
Chinese Journal of Infectious Diseases
2011;29(7):410-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the plasma hepatitis C virus(HCV)RNA levels detected by the fully automated viral load detection system(COBAS TaqMan)and the national real-time quantitative polymerase chain reaction(PCR)kit,and to investigate the clinical application value of these two methods in clinical practice.Methods A total of 168 serial plasma samples collected from 26 patients with chronic hepatitis C(CHC)before and at week 2,4,12,24,36 and 48 of antiviral treatment were detected by both COBAS Taqman 48 analyzing system and the national real-time quantitative PCR kit.The results of two methods were compared by chi square test and t test.Resnlts Both COBAS and national kit showed great positive detecting results when HCV RNA≥1×104IU/mL(at week O),and the virus load value detected by national kit was significantly higher than that detected by COBAS(t=2.05,P<0.05).However,when HCV RNA<1×104(at week 2-48),the positive rate of HCV detected by COBAS was significantly higher than that detected by national kit (t=3.66,P<0.01).At week 4 of treatment,the rapid virological response(RVR)rate was 46.2 % (12/26)detected by COBAS,while that was 88.5%(23/26)detected by national kit,and the difference was significant(x2=10.575,P<0.01).At week 12 of treatment,the complete early virological response(cEVR)was 95.7%(22/23)detected by COBAS,while that was 100%(17/17)detected by national kit,and the difference was not significant(x2=0.726,P>0.05).Conclusions The national TaqMan real-time quantitative PCR kits could be used to screen the suspected cases of HCV infecrion and to diagnose CHC cases with high HCV virus load.COBAS detection is more sensitive in cases with low HCV virus load and in on-treatment monitor during anti-HCV therapy.
