Identification of a suspected Klebsiella variicola strain isolated from a patient with severe septic shock
10.3760/cma.j.issn.1000-6680.2011.06.002
- VernacularTitle:严重感染性休克患者血中分离的一株Klebsiella variicola菌株的鉴定
- Author:
Hongping WANG
;
Peiyan YE
;
Wenjuan WU
;
Xiaoming ZHOU
- Publication Type:Journal Article
- Keywords:
Klebsiella;
Shock,Septic;
Cross infection;
Gene amplification;
Sequence analysis;
Evolution,molecular
- From:
Chinese Journal of Infectious Diseases
2011;29(6):325-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify a suspected Klebsiella variicola strain isolated from a patient with severe septic shock. Methods Sequences of rpoB, gyrA, mdh, infB, phoE and nifH genes were obtained from the clinical isolated suspected Klebsiella variicola strain, and used for constructing phylogenectic trees. Characteristics of phylogenetic trees were used to distinguish Klebsiella variicola from Klebsiella pneumoniae. Results All target gene sequences from this clinical isolated strain constantly formed a cluster with their counterparts from other Klebsiella variicola strains. These clusters were readily differentiated from Klebsiella pneumoniae clusters. Conclusions The clinical isolated strain belongs to Klebsiella variicola class and can cause septic shock. From genetic perspective, Klebsiella variicola is distinctly different from Klebsiella pneumoniae, most of which can be isolated from normal healthy people.
