Mixed culture of Madin-Darby canine kidney cells, human epidermoid cancer cells and African green monkey kidney cells for detection of common respiratory viruses and enteroviruses
10.3760/cma.j.issn.1000-6680.2011.06.001
- VernacularTitle:犬肾细胞系、人喉表皮癌细胞、非洲绿猴肾细胞混合培养检测常见呼吸道病毒和肠道病毒
- Author:
Yingyang GAO
;
Chenyan JIANG
;
Lufang JIANG
;
Qianli WANG
;
Liwen JU
- Publication Type:Journal Article
- Keywords:
Cells,cultured;
Respirovirus;
Enterovirus;
Kidney;
Cell line;
Larynx
- From:
Chinese Journal of Infectious Diseases
2011;29(6):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a clinical test assay for detecting common respiratory viruses and enteroviruses (EV) by using mixed cultured Madin-Darby canine kidney cells (MDCK), human epidermoid cancer cells (Hep-2) and African green monkey kidney cells (Vero) to isolate common respiratory viruses and enteroviruses. Methods Throat swabs with influenza A and B viruses,adenovirus and EV71 were incubated with mixed cultured MDCK, Hep-2 and Vero in a single vial to observe the presence of cytopathic effects. Polymerase chain reaction (PCR), reverse transcription (RT)-PCR and monoclonal antibody-based immunofluorescene assay were also used for confirmatory test. Results The sensitive cell lines developed obvious cytopathic effects to the corresponding viruses, which were confirmed by the specific green particles observed by immunofluorescence assay and specific target PCR segments. Conclusions The shell-vial of mixed cells can simultaneously isolate different common respiratory viruses and EV. The isolated pathogens can be further confirmed by antigen test and PCR. This assay may improve the diagnosis of clinical viral diseases.
