Expressions and significance of interferon gamma produced by natural killer cells in patients with chronic hepatitis B
10.3760/cma.j.issn.1000-6680.2011.05.009
- VernacularTitle:慢性乙型肝炎患者自然杀伤细胞干扰素γ的表达及其与病情的关系
- Author:
Xiaojuan YANG
;
Guorong WU
;
Lihua HUANG
;
Zhonghua LU
- Publication Type:Journal Article
- Keywords:
Hepatitis B,chronic;
Liver;
Killer cells,natural;
Flow eytometry;
Interferon type Ⅱ
- From:
Chinese Journal of Infectious Diseases
2011;29(5):292-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of natural killer cells (NK cells) in patients with chronic hepatitis B (CHB). Methods Blood samples and liver biopsies from 108 CHB patients including 43 mild, 28 moderate and 37 severe CHB patients were collected. The levels of interferon (IFN) gamma in liver tissues and peripheral blood were detected by intracellular cytokine staining.The data were analyzed by t test. Results The levels of IFN gamma produced by NK cells in liver tissues and peripheral blood of patients with severe CHB were (8. 12±4. 26) % and (4. 59±2. 95) % ,respectively, which were significantly higher than those in patients with mild CHB [(3. 53±1. 56)%and (2. 26±0.61)%, respectively; t=3. 80 and t = 4. 30, respectively; both P<0. 05] and those in patients with moderate CHB [(4. 59±1. 98)% and (2. 48±1. 05)%, respectively;t=2. 62 and t =3. 28, respectively; both P<0. 05]. While there were no differences of IFN gamma produced by NK cells both in liver tissues and peripheral blood between mild and moderate CHB patients (t=0. 99 and t=1. 27, respectively; both P>0. 05). The levels of IFN gamma produced by NK cells in liver tissues and blood samples in severe phase of severe CHB were (10. 02 ± 4. 15)% and (6. 78 + 2. 91)%, respectively, those in recovery phase were (6. 13±2. 01)% and (3. 13± 1. 52)%, respectively (t = 1. 89 and t=1. 74, respectively; both P<0. 05]. Conclusion NK cells may play a pivotal role in the pathogenesis of severe CHB.
