Association of mannose binding lectin genetic polymorphisms with cryptococcosis
10.3760/cma.j.issn.1000-6680.2011.05.004
- VernacularTitle:甘露糖结合凝集素基因多态性与隐球菌病易感性的遗传关联研究
- Author:
Xueting OU
;
Jiqin WU
;
Liping ZHU
;
Qiangqiang ZHANG
;
Feifei WANG
;
Bin XU
;
Xiuping HU
;
Xuan WANG
;
Ruiying WANG
;
Xinhua WENG
- Publication Type:Journal Article
- Keywords:
Mannose;
Agglutinins;
Cryptococcosis;
Meningitis,cryptococcal;
Polymorphism,genetic;
Genetic predisposition to disease
- From:
Chinese Journal of Infectious Diseases
2011;29(5):270-275
- CountryChina
- Language:Chinese
-
Abstract:
Objective To describe the distribution of mannose binding lectin (MBL) genetic polymorphisms in non-acquired immunodeficiency syndrome (AIDS) patients with cryptococcosis in China and to verify the association of MBL polymorphisms with susceptibility to cryptococcosis.Methods The case-controlled genetic association study was conducted and 167 non-AIDS patients with cryptococcosis and 208 healthy controls were recruited. Genome DNA was extracted from the peripheral blood and MBL gene was amplified by polymerase chain reaction (PCR). Six singlenucleotide polymorphisms ( SNP) of MBL gene were sequenced. The association of MBL polymorphisms with susceptibility to cryptococcosis were analyzed. The comparison between patients and controls was performed by chi square test or Fisher's exact test. The differences of MBL plasma concentrations between groups with different MBL genotypes were compared by single factor variance analysis. Results There were no differences between patients and controls in terms of MBL genotype frequencies, haplotypes and genotypes (all P>0. 05). Compared with healthy control, the deficient MBL-producing genotypes were strongly associated with cryptococcal meningitis (16. 5% vs 8. 7%,χ2=4.25, P=0.0392, OR = 2.09), particularly in patients without underlying immunocompromised conditions (21. 4% vs 8. 7%, χ2 =7. 15, P = 0. 0075, OR = 2. 88). Individuals with MBL deficiency genotypes showed significantly higher rates of central nervous system (CNS) cryptococcal infection rather than non-CNS cryptococcosis (16. 5% vs 3. 1%, Fisher's exact test, P = 0. 010, OR = 6. 13).The difference was even more significant in the immunocompetent patients (21. 4% vs 4. 0%, P =0.009, OR= 6. 55). Conclusion MBL deficiency is associated with cryptococcal meningitis and may play a role in CNS Cryptococcus infection.
