Preparation, identification and application of polyclonal antibody against major outer membrane protein of Chlamydia trachomatis E
10.3760/cma.j.issn.1000-6680.2011.05.001
- VernacularTitle:E型沙眼衣原体主要外膜蛋白多克隆抗体的制备、鉴定和初步应用
- Author:
Yuanjun LIU
;
Caihong SHENG
;
Yong LIU
;
Mei WANG
;
Qiuyan YANG
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Bacterial outer membrane proteins;
Antibodies,bacterial;
Glutathione transferase;
Enzyme-linked immunosorbent assay;
Blotting,Western;
Fluoroimmunoassay
- From:
Chinese Journal of Infectious Diseases
2011;29(5):257-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express major outer membrane protein (MOMP) of Chlamydia trachomatis E and to prepare rabbit polyclonal antibody. Methods The recombinant plasmid MOMP/ pGEX6p-l prepared by our lab was introduced into E. coli. The protein was expressed and purified by gel recycling, then was injected into New Zealand rabbits to produce polyclonal antibodies. Enzyme linked immunosorbent assay (ELISA) was used to detect the titer of antibody. The antibody specificity was identified by Western blot and immunofluorescence. Results The fusion recombinant protein glutathione S-transferase (GST)-MOMP was successfully expressed in E. coli. The titer of antibody recombinant protein detected by Western blot and to the endogenous MOMP of Chlamydia trachomatis in vitro detected by immunofluorescence. Conclusions The recombinant MOMP is successfully expressed and the MOMP antibody with high titer and high specificity is obtained. which will be helpful for Chlamydia trachomatis detection and related clinical research.
