Pathological ultrastructure changes of lung tissue in diabetic rat and the preventive effect of rosiglitazone intervention
10.3760/cma.j.issn.1008-6315.2011.07.011
- VernacularTitle:糖尿病大鼠肺组织超微结构病理变化及罗格列酮干预研究
- Author:
Weixin MU
;
Yaxin SHEN
;
Hong ZHOU
;
Jing HUANG
;
Mian WANG
;
Guang LIU
- Publication Type:Journal Article
- Keywords:
Diabetic;
Rat;
Ultrastructure;
Pulmonary fibrosis;
Rosiglitazone
- From:
Clinical Medicine of China
2011;27(7):700-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the pathological infrastructure changes of lung tissue in diabetic rat with pulmonary fibrosis and the preventive effect of rosiglitazone intervention. Methods The experimental type 2 diabetic rats were yielded by high-sugar, high fat diet plus intraperitoneal injecting streptozotocin (STZ). The pathological infrastructure changes of lung tissue were observed by transmission electron microscopy in the control, diabetes mellitus group and rosiglitazone treatment group respectively, and the hydroxyproline contentswere determined simultaneously. Results We observed accumulation of collagen fibers and extracellular matrix in the lung tissue of simple diabetic rat, which indicated pulmonary fibrosis. The hydroxyproline contents were significantly higher in diatetes mellitus group when compared to the control at 12 weeks[ 0. 77 ± 0. 03μg/(mg·prot) vs.0.63 ±0.03 μg/(mg·prot)] and 20 weeks [0.93 ±0. 10 μg/(mg·prot) vs.0.87 ±0.10μg/ (mg·prot) ]. (Ps < 0.05) , but the rosiglitazone intervention significantly reduced the hydroxyproline 3contents in diabetes mellitus rats [0.85 ±0.08 μg/(mg·prot) vs.0.87 ±0. 10 μg/(mg·prot) ,P<0.05].Conclusion Pulmonary fibrosis occurred in diabetic rats lung tissue,while rosiglitazone can alleviate it.
