The effect of let-7b and miR-199a on B16F10 cell growth and proliferation
10.3760/cma.j.issn.1008-1372.2011.06.001
- VernacularTitle:Let-7b和miR-199a对B16F10细胞增殖生长的影响
- Author:
Jianda ZHOU
;
Jianxiang TAN
;
Huiqing XIE
;
Bimei JIANG
;
Ming ZHOU
;
Haihang LIU
;
Ming LI
;
Yanwen ZHANG
;
Dan XU
;
Jiye CHEN
;
Xiong LI
;
Chengqun LUO
- Publication Type:Journal Article
- Keywords:
MicroRNAs/PD;
Melanoma/PA/DT;
Cell proliferation;
Cell growth processes
- From:
Journal of Chinese Physician
2011;13(6):721-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm whether or not let-7b and miR-199a were significantly associated with malignant melanoma growth and proliferation. Methods An over -expression plasmid and an inhibitor, which targeted on let-7b and miR-199a, was constructed. B16F10 cells were divided into seven groups: control group, let-7b plasmid group, miR-199a plasmid group, empty plasmid group, let-7b inhibitor group, miR-199a inhibitor group, inhibitor control group. Foreign gene was transfected into B16F10 cells, let-7b and miR-199a expression were validated from RNA level, protein level and cell level. Results The relative let-7b or miR-199a gene expression of the let-7b plasmid group (3.8776±0.1372)and miR-199a plasmid group (2.8660±0.2821)were significantly higher than control group (P＜0.05), the relative let-7b or miR-199a gene expression of the let-7b inhibitor group (0.2057±0.0263) and miR-199a inhibitor group(0.2656±0.0253) were significantly lower than control group(P＜0.05). The cyclinD1 expression of the let-7b plasmid group(2.023±0.315) and let-7b inhibitor group (1.857±0.377) were significantly higher than control group (0.997±0.041) (P＜0.05), whereas, the Met expression of themiR-199a plasmid group (5.19±0.309) and miR-199a inhibitor group (4.87±0.044) were significantly higher than control group (2.2±0.198) (P＜0.05). The let-7b plasmid group and miR-199a plasmid group B16F10 cell growth rate were slower than control group, especially on the third day after transfection, the growth rate gradually dropped to the lowest value (P＜0.05). In addition, the apoptosis rates of the let-7b plasmid group and miR-199a plasmid group reach to (11.8±1.19)% and (11.3±1.59)%,which were significantly higher than control group (P＜0.05). Conclusions let-7b and miR-199a may be a negative regulator on the B16F10 cell growth and proliferation.
