Research on the apoptosis of malignant melanoma cell induced by transmembrane form of human blood group A mimotope vaccine
10.3760/cma.j.issn.1008-1372.2011.05.014
- VernacularTitle:跨膜型血型A抗原模拟肽疫苗诱导黑色素瘤细胞凋亡的实验研究
- Author:
Dongzhi CEN
;
Xufeng LI
;
Jianjun ZOU
;
Min LUO
;
Jiren ZHANG
- Publication Type:Journal Article
- Keywords:
Blood group antigens/BI;
Vaccines,subunit/BI/PD;
Melanoma/DT;
Apoptosis/DE
- From:
Journal of Chinese Physician
2011;13(5):624-626
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptotic effect of the transmembrane form vaccine of human blood group A mimotope on malignant melanoma cell line B16. Methods B16 cells were transfected with different recombinant plasmid through Lipofectamine 2000 and incubated with different concentration of monoclonal anti-A antibody at 2.5 μg/ml, 5 μg/ml,10 μg/ml and 20 μg/ml. Apoptosis rate of cells was determined with Annexin Ⅴ/PI double staining by flow cytometry. Results Apoptosis rate to P/F-M-pIRES group B16 cells was 74.74% when anti-A monoclonal antibody concentration was 10 μg/ml; apoptosis rate of plasmids carrying peptide/Fas fusion gene such as P/F-M-pIRES group and P/F-pIRES group were significantly higher than M-pIRES group and pIRES group. The apoptosis rate was statistically significantly different between different recombinated plasmid groups (F＝669.707,P＜0.01). The apoptosis rate was statistically significantly different between different antibody groups (F＝106.596,P＜0.01). The interaction between recombinated plasmid groups and antibody groups was statistically significant (F＝34.806,P＜0.01). Conclusions The transmembrane form vaccine of human blood group A mimotope could induce B16 cell apoptosis in vitro. This vaccine may be a promising candidate for potential malignant melanoma therapy.
