Experimental research of immunophenotype CD133,CD34,CD44 in human lung adenocarcinoma

VernacularTitle:人肺腺癌肿瘤细胞免疫表型CD133、CD34、CD44的实验研究
Author: Sichuang TAN ; Ruoxin WANG ; Sipin TAN ; Wen HU ; Yuchao MA ; Fenglei YU
Publication Type:Journal Article
Keywords: Lung neoplasms/IM; Tumor stem cell assay; Antigens,CD34/ME; Antigens,CD44/ME; Antigens,CD/ME
From: Journal of Chinese Physician 2011;13(5):592-595
CountryChina
Language:Chinese
Abstract: Objective To validate the possibility of CD133 CD34 CD44 be served as biomarkers in cancer stem cell of human lung adenocarcinoma. Methods Two kinds of culturing methods were performed to generate adhesive tumor cells and floating aggregates, and the differences of expression of CD133 CD34 CD44 between 2 kinds of cultured cells were observed by immunofluorescence. Results Floating aggregates grew more slowly, kept activity for longer period than adhesive cells (72.5% vs 47.5%,P＜0.05). Floating aggregates expressed higher level of CD133, CD34 and CD44 than adhesive cells (68.97%,82.76%,93.10% vs 5.26%,15.79%,5.26%,P＜0.01). Conclusions The combination of CD133, CD34 and CD44 probably can be used as surface markers of cancer stem cells for human lung adenocarcinoma.