Effect of butylphthalide on H2S content and the expression of NR2B in the hippocampus of alcohol dependence rats
10.3760/cma.j.issn.1674-6554.2011.06.004
- VernacularTitle:丁苯酞对酒精依赖大鼠海马硫化氢含量及NMDA受体2B亚基表达的影响
- Author:
Ailin DU
;
Chunyang XU
;
Hongbo JIANG
;
Kun SHAO
;
Lu CHEN
;
Fujia HOU
;
Ruiling ZHANG
- Publication Type:Journal Article
- Keywords:
Butylphthalide;
Alcohol dependence;
H2S;
NMDAR2B
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(6):491-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of butylphthalide (NBP) on H2S content and the expression of NR2B in the hippocampus of alcohol dependence rats. Methods A total of 84 SD male rats were randomly divided into 6 groups. Except for the normal group, other groups were subjected to alcohol solution with concentration of 6% ( V/V) for 28 d. Drug intervention began at the 14th day,and rats in the low,medium,high dose group were treated with NBP with a different concentration. Erden abstinence scoring was used to evaluate the rats withdrawal symptom. H2S content was measured in one side of hippocampus and CBS activity was tested in the other side of hippocampus. Hippocampus of 3 rats from each group was used to investigate NR2B mRNA level. Results Withdrawal symptom score ( 12.27 ± 1. 19),H2S content(30. 25 ±8.82), CBS activity (72. 44 ±7. 46) and NR2B mRNA expression( 19. 47 ±0. 86) in medium dose NBP group rats were lower than withdrawal symptom score(14.09 ±2.21) ,H2S content(44. 50 ±6. 65) , CBS activity(79. 06 ±4. 57) and NR2B mRNA expression (29. 13 ±1.39) in experimental control group (P<0.05). Withdrawal symptom score(12. 18 ±1.08) ,H2S content(33.00 ±5.38) ,CBS activity(67. 81 ±9. 37) and NR2B mRNA expression(23. 12 ± 1. 86) in high dose NBP group rats were lower than experimental control group (P < 0. 05). Conclusion NBP can reduce withdrawal symptoms of alcohol dependence rats,may be related to decreased expression of H2S/CBS system, and NR2B mRNA expression.
