Studies on fingerprint of bidens bipinnata L.by HPLC
10.3760/cma.j.issn.1008.6706.2011.14.002
- VernacularTitle:鬼针草药材高效液相色谱法指纹图谱的初步研究
- Author:
Liju ZHANG
;
Wei HU
;
Feihu CHEN
- Publication Type:Journal Article
- Keywords:
Bidens;
HPLC;
Fingerprint
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(14):1875-1877
- CountryChina
- Language:Chinese
-
Abstract:
Objective The fingerprint method based on high-performance liquid chromatography for the quality control of bidens bipinnata L were firstly described and optimized. Methods HPLC system was used to obtain the fingerprint spectrum of bidens bipinnata L. The chromatography condition was ODS C18 column (4. 6mm×255mm,25μm),mobile phase Methanol-0. 2% HAc,and flow speed 1. 0ml ? min-1, detection wavelength 363nm.Results In this experiment,the standard fingerprint had been established and the similitude degree of 10 batches bidens bipinnata L. has also been obtained(0.941～0.999). Conclusion The method was accurate,reliable,good reproducibility, strong characteristic and could be used for the standard of general judgement and the quality estimation about the bidens bipinnata L.
