Preliminary study of cerebral oxygen metabolism and change of blood lactate in early stage of sepsis in rats
10.3760/cma.j.issn.1671-0282.2011.05.018
- VernacularTitle:早期脓毒症大鼠脑氧代谢与乳酸的变化
- Author:
Xin QIAN
;
Luojia TANG
;
Zheng ZHENG
;
Feng CHEN
- Publication Type:Journal Article
- Keywords:
cerebral oxygen metabolism;
jugular vein blood oxygen saturation;
Arteriovenous oxygen content difference;
oxygen extraction fraction;
lactate;
sepsis;
lipopolysaccharide;
blood gas analysis
- From:
Chinese Journal of Emergency Medicine
2011;20(5):511-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of cerebral oxygen metablism and the level of blood lactate in early stage of sepsis in rats.Method Sixty-four SD rats were randomly(random number)divided into septic group and control group.The sepsis model of rat was made by lipopolysaccharide (LPS,10 mg/kg)injected intra-abdominally,and rats of control group were treated with the same amount of physiological saline instead.And each group was further divided into 4 sub-groups of4 h,6 h,12 h and 24 h after treatment.At each interval,blood samples were obtained via jugular vein for detecting blood oxygen saturation (Sjv02)and blood lactate(LA).The blood oxygen saturation(Sa02)of ventral aorta was also measured.Arteriovenous oxygen content difference (AVD02) and oxygen extraction fraction (OEF) were studied.These four variables were analyzed and compared between two groups.Results The AVD02 and OEF in sepsis group were higher than those in control groups of 3 h,6 h and 12 h (P<0.05) ,but lower than those in control group of24 hours (P<0.05).The Sjv02 in sepsis group was lower than that in control groups of 3 h,6 h and 12 h (P<0.05),but increased to that in control group of 24 h (P>0.05).LA in sepsis group was higher than that in control group in each interval (P<0.05).Conclusions In early stage of sepsis,cerebral perfusion,cerebral oxygen supply and cerebral oxygen extraction increase.The global oxygen metabolic variables(Sjv02,AVD02 and OEF)and the level of LA can be used to objectively and accurately evaluate the cerebral oxygen metabolic dysfunction in early stage of sepsis.
