Effect of dexmedetomidine on serum concentrations of TNF-α and IL-1β in acute lung injury following blunt chest trauma in rats
10.3760/cma.j.issn.1671-0282.2011.05.010
- VernacularTitle:右美托咪定对大鼠胸部撞击性肺损伤血清肿瘤坏死因子-α和白介素-1β的影响
- Author:
Mingtao LI
;
Xiaojing WU
;
Zhongyuan XIA
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Blunt chest trauma;
Acute lung injury;
Tumor necrosis factor-α;
Interleukin-1β
- From:
Chinese Journal of Emergency Medicine
2011;20(5):485-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dexmedetomidine on the outcome of rats with acute lung injury following blunt chest trauma.Method Forty male SD rats weighing 250～300 g were randomly (random number)divided into 5 groups(n=8 each),namely,group C(normal),group D(normal plus dexmedetomidine),group T(trauma),group TD(trauma plus dexmedetomidine),group TDY(trauma plus dexmedetomidine plus yohimbine).Au rats were sacrificed by using exsanguination from arteria femoralis 6 hours later.The TNF-α and IL-1β levels in plasma were examined by using ELISA.Lung wet/dry(W/D)weight and the percentage of neutrophil in leucocytes in bronehoalveolar lavage fluid(BALF)of rats were detected.HE staining was performed to observe the injury of lung tissue under microscope.Results There was significant lung injury after blunt chest trauma.The serum concentrations of TNF-α and IL-1β,PMN%and lung wet/dry(W/D)weight were significantly higher in traumatic group(P<0.05,P<0.01).chest trauma,but this protective effect of dexmedetomidine could be blocked by yohimbine,at least in part,via the inhibition of α2-adrenergie receptor.Conclusions Dexmedetomidine has a certain protective effect on acute traumatic acute lung injury in rats.
