Effect of Jiawei-Sini Dection on expression of transforming growth factor-β1 receptor Ⅰ、Ⅱ of hepatic fibrosis in rats
10.3760/cma.j.issn.1673-4246.2011.08.005
- VernacularTitle:加味四逆散对肝纤维化大鼠肝组织TGFβ1受体Ⅰ、Ⅱ表达的影响
- Author:
Lifeng WANG
;
Shoucai SUN
;
Changqin LI
;
Hongyong ZHANG
;
Xurui ZHENG
;
Ning CAO
- Publication Type:Journal Article
- Keywords:
Jiawei-Sini Dection;
Hepatic fibrosis;
TβRⅠ,TβR Ⅱ
- From:
International Journal of Traditional Chinese Medicine
2011;33(8):686-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Jiawei-sini Dection on expression of rat transforming growth factor-β1 receptor Ⅰ,Ⅱ(TβRⅠ,TβR Ⅱ), and study its treatment of anti-HF and the possible mechanisms. Methods The model of rat hepatic fibrosis was setup by subcutaneous injection of carbon tetrachloride and drinking alcohol freely;According to random block method, the successful model rats were divided into three groups:pathological model group (group B),Fufangbiejiarangan tablet group (group C), and Jiawei Sini Decoction group (group D), each group containing ten rats. Group A and group B were given two milliliters saline, group C was given Fufangbiejiarangan tablets 0.625 grams per kilogram of body weight, group D was given Jiawei Sini liquid 15.625 grams per kilogram of body weight.Each rat was fed once a day for 8 weeks.In order to avoid the natural repair of the hepatic affecting the experimental results, the rats,except group A, were still injected 40% CCl4 three milliliters per kilogram of body weight after feeding drug once a week. Fufang-Biejia-Ruangan Ttablet group was set as a positive control;The effects on expression of TβRⅠ,TβR Ⅱ were determined by immunohistochemical method. Changes of alanine aminotransferase (ALT), aspartate aminotransferase(AST),alkaline phosphatase(ALP) and TβRⅠ,TβR Ⅱ were observed in rats. Results The expression of TβRⅠ、Ⅱ, compared with the pathological model(16.63±2.69)%, (14.57±1.09)%, were significantly reduced in Jiawei-Sini Dection group[they are(8.09±0.71)%,(6.51±0.48)%, the difference was statistically significant(P＜0.05).The effects of Jiawei-Sini Dection was equal to the effect of Fufang-Biejia-Ruangan Tablets on expression of TβRⅠ,TβR Ⅱ(P＞0.05). Conclusion Jiawei-Sini Dection was able to inhibit the expression of TβRⅠ and TβR Ⅱ, thus affected the combination of TGFβ1, and their receptors.
