Effects of Huazhuo-Jiedu-Huoxue-Tongluo recipe on the level of IL-6 and TNF-α in mice with cerebral ischemia reperfusion injury
10.3760/cma.j.issn.1673-4246.2011.07.006
- VernacularTitle:化浊解毒活血通络方对脑缺血再灌注损伤小鼠血清IL-6和TNF-α水平的影响
- Author:
Jianwen ZHAO
;
Junbiao TIAN
;
Diangui LI
;
Yanwei ZHANG
;
Lijing YANG
- Publication Type:Journal Article
- Keywords:
Huazhuo Jiedu Huoxue Tongluo recipe;
Cerebral ischemia Reperfusion injury;
Inflammatory reaction;
IL-6;
TNF-α
- From:
International Journal of Traditional Chinese Medicine
2011;33(7):594-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Huazhuo-Jiedu-Huoxue Tongluo recipe on local inflammation of cerebral ischemia reperfusion injury in mice. Method Fifty Kunming Mice were randomly divided into five groups: model group, high-dose group of TCM, low-dose group of TCM, nimodiping group and sham operation group. Each group had 10 mice. Mice models of the right middle cerebral artery CIRI in the first four groups were established through thread embolism method. Physiological saline was given to the mice in the model group and sham operation group. Nimodipine solution was given to the mice in the Nimodipine group. High and low dose of Huazhuo-Jiedu-Huoxue-Tongluo Chinese medicine decoction were given to the mice in the high dose of TCM group and low dose of TCM group respectively after the models were successfully established. To assess the expression level of IL-6 and TNF-α by radioimmunoassay after the treatment. Results Compared with model group [(219.29±39.28) pg/ml, (1.325 ± 0.236) ng/ml] , the level of IL-6 and TNF-α of high dose of TCM group[(120.69±49.23)pg/ml, (1.086±0.178)ng/ml], low dose of TCM group[(173.97±49.03)pg/ml, (0.937 ± 0.105)ng/ml]and nimodipine group[(170.88± 47.06)pg/ml, (1.092±0.184) ng/ml]decrease (P<0.05). There is no significant difference between the high dose of TCM group and the sham operation group (P>0.05). Conclusion Huazhuo-Jiedu-Huoxue-Tongluo recipe can restrain the expression of inflammatory cytokines and reduce the infiltrating of leukocytes in mice with cerebral ischemia reperfusion injury.
