Epidermal growth factor receptor mutations in non-small cell lung cancer of tissue and peripheral blood by fluorescence quantitive polymerase chain reaction

VernacularTitle:实时荧光定量PCR检测非小细胞肺癌患者外周血和肿瘤组织表皮生长因子受体突变的研究
Author: Jin YANG ; Zhonghe YU ; Huilong LIU ; Chunhong HAN
Publication Type:Journal Article
Keywords: Carcinoma,non-small cell lung; Receptor,epidermal growth factor; Protein-tyrosine kinase; Mutation; Fluorescence quantitive polymerase chain reaction
From: Journal of International Oncology 2011;38(6):468-471
CountryChina
Language:Chinese
Abstract: Objective To investigate gene mutations of the epidermal growth factor receptor of tumor tissue and peripheral blood in non-small-cell lung cancer, and to compare the consistency of EGFR mutations in tumor tissue and peripheral blood, fluorescence quantitive polymerase chain reaction (FQ-PCR) was performed according to standard procedure. Methods 112 peripheral blood and 87 tumor tissue of NSCLC were detected by FQ-PCR, among which 45 cases were analyzed using peripheral blood-tumor tissue matched samples. The relationship between clinical efficacy and EGFR mutations Was analyzed. Results Our results showed that there is 32 mutations in EGFR in 112(28.6%)of the patients'peripheral blood samples and 27 mutations in EGFR in 87(31.O%)tumor tissue. EGFR mutations were mainly found in adenocarcinoma and non-smokers in NSCLC (P<0.05). The consistency of EGFR mutation between tumor tissue and peripheal blood reached 71.4%. The clinical outcome of mutations Was significantly effective(P