Study the expression of caspase-3 on trehalose as cryoprotectant for preserving aortic valve homograft in liquid nitrogen
10.3760/cma.j.issn.1673-4203.2011.06.009
- VernacularTitle:海藻糖对液氮深低温保存的同种瓣caspase-3表达的影响
- Author:
Chenchen CHENG
;
Qing CHANG
;
Ping XU
;
Hongbo GAO
- Publication Type:Journal Article
- Keywords:
Trehalose;
Cryopreserved;
Allograft valve;
Caspase-3
- From:
International Journal of Surgery
2011;38(6):386-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of caspase-3 on the trehalose as cryoprotectant for preserving aortic valve homograft in liquid nitrogen.Methods The aortic valve homograft was divided into 5groups,namely:0.1 mol/L DMSO(control group),0.1 mol/L trehalose(experimental group 1),0.1 mol/L trehalose+0.1 mol/L DMSO(experimental group 2),0.2 mol/L trehalose+0.1 mol/L DMSO(experimental group 3),0.3 mol/L trehalose+0.1 mol/L DMSO(experimental group4).At the time of 12 months,15 months and 18 months when preserved in liquid nitrogen,relative expression of caspase-3 of the aortic valve homograft was measured by RT-PCR and Western Blot.Fresh group was a negative control group.Results At the same time(P<0.05),the expression of caspase-3 of fresh aortic tissue was slightest.The experimental group 2 was in accord with the experiment group 3,which was of a sort compare with the fresh group.The experimental group 4,which was worse than the experimental group 2 and 3,ranked above the experimental group 1.The worst was the control group.Conclusions The joint use of trehalose and DMSO could well inhibit the expression of caspase-3.Moreover.0.1mol/L trehalose+0.1 mol/L DMSO and 0.2 mol/L trehalose +0.1 mol/L DMSO could maximize the inhibition of the expression of caspase-3.
