Proteomics analysis of Sut melanocytes in response to xCT deficiency
10.3760/cma.j.issn.1673-4181.2011.03.003
- VernacularTitle:xCT缺陷的Sut黑色素细胞蛋白质组学研究
- Author:
Liming SHI
;
Liping ZHAO
;
Lu HE
;
Wenhui YAN
;
Haixuan QIAO
- Publication Type:Journal Article
- Keywords:
Proteomics;
Sut melanocytes;
xCT
- From:
International Journal of Biomedical Engineering
2011;34(3):140-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Sut melanocytes growth inhibition in response to xCT -deficiency. Methods TTotal proteins were extracted from xCT-deficient Sut melanocytes and wild melanocytes, respectively, and were separated by 2-dimensional electrophoresis. Altered expression profile of proteins of Sut melanocytes was analyzed by PDQuest software and compared with that of wild melanocytes.Proteins with significant change were chosen to be identified by mass spectrometry and database query.Results Twenty proteins in Sut melanocytes altered significantly compared with wild melanocytes. Ten of the proteins were up-regulated, while the other tens were down-regulated. Four proteins from both up-regulated and down-regulated were identified respectively: up-regulated proteins were Tubulin alpha-1b, S100-A6,Nucleoside, S-formylglutathione, and down-regulated proteins were Calumenin,NDRG1 ,DPYSL2, 14kDa unknown protein. Conclusion The identification of the xCT-deficiency related proteins may provide supporting evidence for the mechanism research of Sut melanocytes' growth inhibition caused by xCT-deficiency.
