Fabrication of tissue engineered trachea by in vitro and in vivo culturing of chondrocytes in plga or dacron porous scaffolds

VernacularTitle:三维支架与肋软骨细胞体外及体内培养构建组织工程化气管
Author: Zhengcheng LIU ; Xiang GUO ; Heng ZHAO
Publication Type:Journal Article
Keywords: Tissue engineer; Trachea; Stents; Cartilage; Tissue culture; Animal testing alternatives
From: Chinese Journal of Thoracic and Cardiovascular Surgery 2011;27(7):432-436
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of a tissue engineered trachea for replacement fabricated using three dimensional scaffold and chondrocytes by in vitro and in vivo culturing. Methods Rib chondrocytes were isolated and expanded to two passages, then seeded in PLGA or Dacron scaffold at density of 5 × 107/ml. Cultured in vitro for two weeks, the chondrocytes-scaffold model was planted under dorsal skin between nude mice's spine. Histology of cartilage, neovascularization and organizational structure were observed with HE staining, PAS staining and electron microscopic scan were performed after 4,6,8 weeks in vivo. Results Organized structure were observed in both PLGA-chondrocyte model and dacron-chondrocyte model with cartilage formation, neovascularization and tight fibrous connective tissue between scaffold and skin after in vitro and in vivo culture. Conclusion Tissue engineered trachea fabricated using rib chondrocytes and PLGA or dacron scaffold with in vitro and in vivo culture meets the requirement of trachea replacement.