Quantitative determination of total alkaloids in Lotus Leaves by colorimetry and UV spectrophotometry
- Author:
Nguyen Kim Can
- Publication Type:Journal Article
- Keywords:
total alkaloids, Lotus Leaves
- MeSH:
alkaloids;
Plant Leaves;
Lotus;
Spectrophotometry;
Colorimetry;
- From:Journal of Medicinal Materials - Hanoi
2004;9(3):79-82
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
From the leaf of lotus (Nelumbo nucifera), total alcaloids were extracted and purified in using chloroform in alcaline medium and 3% phosphoric acid solution.In mixing with bromothymol blue in buffer solution at pH=7, the complex was measured at λ =615± 1nm on spectrophotometer. For UV spectrophotometry, total alcaloids in solution were measured at λ=270nm
