Rapid identification of Pseudomonas aeruginosa by proteinchip golden array
10.3760/cma.j.issn.0254-5101.2011.05.018
- VernacularTitle:利用Au蛋白芯片技术快速鉴定铜绿假单胞菌的研究
- Author:
Chunbao XIE
;
Daiwen XIAO
;
Yongchang YANG
;
Wei JIANG
;
Hua LIU
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Protein fingerprinting;
Au proteinchip golden array
- From:
Chinese Journal of Microbiology and Immunology
2011;31(5):462-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish protein fingerprinting identification model of Pseudomonas aeruginosa (P. aeruginosa) and to lay a foundation for rapid identification of P. aeruginosa by proteinchip golden array. Methods Sixty-four P. aeruginosa and one hundred and ninety-nine control bacteria identified in our laboratory were collected and divided into training and testing group. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and proteinchip golden array were used to detect the protein profiling of the bacteria. Data were automatically collected by Ciphergen Proteinchip Software and protein markers of P. aeruginosa were screened by BioMarker Wizard Software. Classification tree model was developed and validated by BioMarker Patterns Software. The model was blindly tested with twenty-nine P. aeruginosa and sixty-four control bacteria. Results Eighty protein peaks were detected between 3000 and 20 000, among which fifty-eight ones showed significantly difference between P. aeruginosa and the control bacteria (P＜0.01). By BioMarker Patterns Software, one protein peak ( M/Z at 14 045.2) was chosen to develop a classification tree model. The results exhibited with sensitivity of 96. 55% and specificity of 100%. Conclusion Proteinchip golden array has the potential for rapid identification of P. aeruginosa.
