Characteristics in frequency and phenotype of myeloid and plasmacytoid dendritic cells in chronic HCV infection
10.3760/cma.j.issn.0254-5101.2011.05.015
- VernacularTitle:慢性HCV感染者外周血中髓样和浆样树突状细胞频数和表型研究
- Author:
Siyong CHEN
;
Hua LIANG
- Publication Type:Journal Article
- Keywords:
Hepatitis C;
Myeloid dendritic cells;
Plasmacytoid dendritic cells;
Flow cytometry
- From:
Chinese Journal of Microbiology and Immunology
2011;31(5):448-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the frequencies and phenotype of myeloid and plasmacytoid dendritic cells (mDC and pDC) in chronic HCV infection and to investigate the relationships between DC frequencies and HCV viral load and serum ALT level. Methods PBMC were isolated from chronic HCV infected patients and healthy control. Multi-color flow cytometry was used to analyze the frequencies and surface marker expression on mDC and pDC. The relationship between DC frequencies and viral load and ALT level was also calculated. Results In comparison with healthy control, frequencies of mDC and pDC in chronic HCV infection were significantly decreased (0. 37 ± 0. 19 and 0. 19 ± 0. 12 vs 0. 51 ± 0. 18 and 0. 29 ± 0.13, P＜0.05). The frequency of mDC was negatively correlated with HCV viral load (r= -0.5878, P ＜ 0. 0001 ) and serum ALT level ( r = - 0. 4628 , P = 0. 003 ). Both costimulatory markers ( HLA-DR, CD83, CD86, and CD40) and coinhibitory marker (PD-L1) expression on mDC and pDC in HCV infection were increased (P＜0.01 for costimulatory marker, P＜0.05 or F＜0.01 for coinhibitory marker). Conclusion The frequencies of mDC and pDC in chronic HCV infection were decreased, while the expression of costimulatory markers and coinhibitory marker were increased or not decreased in HCV infection. The decreased frequency of mDC was probably related to persistance of HCV infection.
