Pretreatment of IL-2 influences the proliferation and polarization of mice splenic lymphocyte following mobilization with G-CSF
10.3760/cma.j.issn.0254-5101.2011.05.010
- VernacularTitle:IL-2预孵育影响G-CSF动员的小鼠脾淋巴细胞增殖及极化观察
- Author:
Hesheng HE
;
Weilian GENG
;
Yanhui XIE
- Publication Type:Journal Article
- Keywords:
Interleukin-2;
Granulocyte colony-stimulating factor;
Cell proliferation;
Cell polarization;
Mixed lymphocyte reaction
- From:
Chinese Journal of Microbiology and Immunology
2011;31(5):429-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of IL-2 pretreatment on splenic lymphocyte following mobilization with G-CSF, which may provide a new approach to attenuate acute graft-versus-host disease (GVHD). Methods Splenic cells and na(i)ve CD4+T cells from C57BL/6N mice receiving G-CSF-mobilized were pretreated with IL-2(50 U/ml) , and cocultured with the allogeneic antigens from BALB/c mice. The proliferation responses and the polarization of T cells were determined. C57BL/6N mice were randomly divided into 4 groups: G-CSF + IL-2 group, G-CSF group, IL-2 group , control group. Results Compared with the control group, IL-4 increased obviously while IFN-γ decreased significantly in the group of G-CSF + IL-2. The proliferation responses were also suppressed in vitro. Conclusion The proliferation responses of splenic cells and naive CD4 + T cells from C57BI/6N mice receiving G-CSF-mobilized to the allogeneic antigens were significantly abrogated by the pretreatment with IL-2, T cells were polarized toward the production of type-2 cytokines. The combination of G-CSF and IL-2 is potentially synergetic in the induction of T lymphocyte immune tolerance.
