Expression of Legionella pneumophila virulence genes under biofilm condition
10.3760/cma.j.issn.0254-5101.2011.05.006
- VernacularTitle:嗜肺军团菌生物膜的形成与毒力基因表达量分析
- Author:
Zhaoyan ZHOU
;
Liang QIN
;
Bijie HU
;
Watanabe HIROSHI
- Publication Type:Journal Article
- Keywords:
LegioneUa pneumophilla;
Biofilm;
Violence gene;
RNA expression
- From:
Chinese Journal of Microbiology and Immunology
2011;31(5):413-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the physiological state of L. pneumophila in biofilm. Methods Genes previously identified as good markers for the transmissive and replicative phases of the L. pneumophila life cycle during growth in Acanthamoeba castellanii were examined for their expression fold change in the sessile cells as compared to planktonic cells using real-time RT PCR. Results Mature L. pneumophila biofilms were formed at 37t in 75 cm2 cell culture treated flasks for 18 days. The ratio of gene (mip, flaA and fliA) expression in post-exponential cells compared to exponential cells is 0. 53, 4. 45 and 3. 67. The exponential phase cultures display replicative traits and post-exponential bacteria express transmissive factors. The ratio of gene (mip, flaA and fliA) expression in sessile cells compared to exponential cells is 4.42, 5.24 and 16.21, while the sessile cells compared to exponential cells is 8.39, 1. 18 and 4. 43, respectively. Conclusion The violence gene expression of L pneumophila in biofilm is unique.
