Host immune responses to avian influenza A H5N1 virus in a BALB/c model
10.3760/cma.j.issn.0254-5101.2011.05.005
- VernacularTitle:禽流感H5N1亚型病毒感染BALB/c小鼠的免疫应答
- Author:
Ren HUANG
;
Shaochang DENG
;
Yingyan WANG
;
Yu ZHANG
;
Yue WU
;
Wende LI
;
Weibo ZHAO
;
Xiangmei LIU
;
Zheng GUO
- Publication Type:Journal Article
- Keywords:
Avian influenza A(H5N1) viruses;
BALB/c mouse;
T lymphocytes;
Cytokine
- From:
Chinese Journal of Microbiology and Immunology
2011;31(5):406-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cell immunity and eytokines responses to avian influenza A H5N1 virus infections in a BALB/c model to better understand the pathogenesis of H5N1 avian influenza disease. Methods Two hundred and twenty BALB/c mice of the infected group were inoculated with 0.1 ml (10-4.875 TCID50) of A/Goose/Guangdong/NH/2003 ( H5N1 ) virus intra-nasally. Fifty control mice received noninfectious allantoic fluid and another fifty control mice received normal sodium. Blood and spleen samples were collected from the live mice every 24 h during the 14 d post-infection. The changes of CD3 + T cells , CD4 + T cells, CD8 + T cells for cell immunity in blood circulation and spleen were detected by flow cytometry. And the cytokines and antibody responses in blood circulation were detected by ELISA. Necropsy was performed on mice that died during the experiment and those euthanized at end of study. Results Avian influenza A( H5N1) virus infections can make damages to the cell immune system transiently. The CD3 + T cells, CD4 + T cells, CDS + T cells declined at 24 days post infection in blood circulation and declined at 5-8 days in spleen, then recovered to the normal level gradually. The eytokines responses to the infections can be detected: the level of IFN-γ,TNF-α declined, IL-4, IL-18, IL-10 increased, and IL-2 changed little. The antibody increased rapidly from day 7 post infection until the end of the study (day 14 post infection). Conclusion Collectively, avian influenza A(H5N1) virus can cause cell immunity deficiency and an imbalance in the level of eytokines, which may contribute to the unusual severity of disease caused by the H5N1 avian influenza virus.
