Comparison of kidney tubular epithelial cells and endothelial cells grown on titania nanotubes
10.3760/cma.j.issn.1001-7097.2011.07.015
- VernacularTitle:肾小管上皮细胞与内皮细胞在TiO2纳米管上种植的对比
- Author:
Huiqiong LIU
;
Wen ZHU
;
Jianfeng LIU
;
Xi LIU
;
Dali TONG
- Publication Type:Journal Article
- Keywords:
Kidney,artificial;
Epithelial cells;
Endothelial cells;
Nanotubes,Titanium;
Cell implantation;
Cell viability
- From:
Chinese Journal of Nephrology
2011;27(7):525-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the adhesion and growth of LLC-PK1 cells and ECV304 cells on titania nanotube arrays, and provide evidence for construction of miniaturation bioartificial kidney. Methods Four different diameters nanotube materials were prepared by anodic oxidation, each material was processed by unannealed and with UV irradiation, annealed and without UV irradiation, annealed and with UV irradiation, respectively, which had 12 groups totally,then two kinds of cells were separately grown on the 12 materials. The adhesion and growth of the two kinds of cells were studied under a fluorescence microscope. MTT assay was used to test the activity of two kinds of cells on different diameters and the proliferation of two kinds of cells on 70 nm diameters. Results The adhesion and proliferation of two kinds of cells on TiO2 nanotube arrays were basically consistent, both on anatase TiO2 nanotubes with 70 nm diameter but without UV irradiation showed the optimal adhesion and activity. The activities of LLC-PK1 cells and ECV304 cells were both increased with time extended, while the absorbance of ECV304 cells was higher on pure Ti film than on titania nanotube. Conclusion TiO2 nanotube is beneficial to LLC-PK1 cells, but is unfavorable for ECV304 cells when they grow alone.
