Change of V-ATPase B subunit in transforming growth factor β1-induced epithelial-mesenchymal transition of rat renal proximal tubular cells
10.3760/cma.j.issn.1001-7097.2011.06.012
- VernacularTitle:囊泡型H+-ATP酶B亚基在转化生长因子β1致肾小管上皮细胞转分化中的变化及其意义
- Author:
Xueqin CAO
;
Shili ZHAO
;
Jing QIN
;
Xiaoyan LI
;
Jinjin FAN
;
Haiping MAO
;
Qiongqiong YANG
;
Xueqing YU
- Publication Type:Journal Article
- Keywords:
Proton-translocating ATPases;
Transforming growth factor betal;
Kidney tubules;
Epithelial cells;
Epithelial to mesenchymal transition;
Vacuolar H+-ATPase
- From:
Chinese Journal of Nephrology
2011;27(6):448-453
- CountryChina
- Language:Chinese
-
Abstract:
Objecfive To investigate the change of V-ATPase B subunits on epithelial to mesenchymal transition (EMT)in rat renal tubular epithelial cells (NRK52E) stimulated by transforming growth factor β1 (TGF-β1). Methods NRK52E cells were stimulated by TGF-β1 (10 μg/L)for O h(control),12 h,24 h,48 h,72 h after sefrum-free culture for 24 h.The mRNA and protein expression of E-cadherin,α-SMA,B2 and B1 subunits of V-ATPase were detected by real-time PCR,Western blotting and immunofluorescence. Results After stimulated by TGF-β1 (10 μg/L)for 48 h,the expression of α-SMA was markedly increased(P<0.05),but the expression of E-cadherin was dramatically decreased(P<0.05).Meanwhile,the expressions of V-ATPase subunit B2 was significantly increased (P<0.05).However,the B1 subunit distributed rarely in NRK 52E cells,and did not increase after TGF-β1 stimulation.Double-label immunofluoerscence staining also showed that the V-ATPase B2 subunit was increased in the cytosol.tending to accumulate to the cell membrane after TGF-β1 stimulation. Conclusions The main isoform of V-ATPase distributed in NRK52E cells is B2 subunit.B2 subunit is increased alone with TGF-β1-induced EMT.It may suggest that V-ATPase B2 subunit may play a potential role in TGF-β1-induced tubular EMT and renal fibrosis.
