Prokaryotic expression and pilot application of capsid proteins of WU polyomavirus
10.3760/cma.j.issn.0254-5101.2011.06.013
- VernacularTitle:WU多瘤病毒衣壳蛋白原核表达及初步分析
- Author:
Yinhui ZHANG
;
Qiong WANG
;
Xue JIA
;
Guangyu LIN
;
Lie HUANG
;
Jian LIU
;
Xuedong LU
- Publication Type:Journal Article
- Keywords:
WU polyomavirus;
Capsid protein;
Prokaryotic expression
- From:
Chinese Journal of Microbiology and Immunology
2011;31(6):528-531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express the capsid proteins of WU polyomavirus(WUPyV) for research and find antigen for diagnostic value. Methods Coding sequences of capsid proteins of WU polyomavirus by PCR were cloned in prokaryotic expression vector PGEX-20T. Recombinant plasmids were transformed into E. coli BL21(DE3) and induced by IPTG for proteins expression. Recombinant proteins were identified by Western blot. Results SDS-PAGE proved that recombinant proteins showed three bands with molecular relative mass of 69×103, 63×103 and 56×103. The recombinant proteins were recognized by anti-GST McAb. The antigenicity was tested by Western blot using 16 WU polyomavirus positive and 70 negative sera. Conclusion Recombinant VP1, VP2 and VP3 expressed in E. coli can combine with WUPyV-Ab and have good antigenicity. They can be used for further research.
