The feature analysis of soluble liver antigen B cell epitopes distribution in autoimmune hepatitis patients
10.3760/cma.j.issn.0254-5101.2011.06.007
- VernacularTitle:自身免疫性肝炎患者中可溶性肝抗原B细胞抗原表位分布特征分析
- Author:
Yan ZHAO
;
Shuang WANG
;
Huiping YAN
;
Dongmei MA
;
Haiping ZHANG
;
Xia FENG
;
Yonghong ZHANG
- Publication Type:Journal Article
- Keywords:
Autoimmune hepatitis;
Soluble liver antigen;
Epitope;
ELISA
- From:
Chinese Journal of Microbiology and Immunology
2011;31(6):507-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the feature of B cell epitope of soluble liver antigen(SLA) in autoimmune hepatitis. Methods Twenty-one autoimmune hepatitis(AIH) were enrolled the study. The control groups included 15 primary biliary cirrhosis(PBC) patients, 20 chronic virus hepatitis patients and 20 heaths. The serum incubated with coated one dimension of SLA peptide pools by ELISA for mapping the B cell epitopes. ELISPOT was performed to screen the SLA specific T cell response by observing the secretion of IFN-γ after PBMC simulated. Results Ten serum samples from AIH patients recognized the SLA peptide pools, the frequency was 47.62%, which was higher than PBC group, virus hepatitis group and normal control group, P value was 0.001, <0.001 and <0.001, respectively. The 10 positive serum dominant response to SLA 7 th pool(aa385-441), which laid in the unique no conservative region of the SLA molecular. T cell recognizing regions laid dominantly in first and second pool, the frequency was 30% and 40%, respectively, however the recognized frequency of 7th pool was 10%. Conclusion B cell linear epitopes lie in the sequence aa385-441, which is the unique unconservative region in the high conservative SLA molecular and overlaps with part sequence of T cell epitopes. The results show it is complex and interaction that SLA induced humoral-mediated immunity and cellular immunity to involve in AIH pathogenesis.
