The role of NF-κB in the differentiation process of dendritic cells induced by astragalus polysaccharides from human cord blood monocyte
10.3760/cma.j.issn.0254-5101.2011.04.004
- VernacularTitle:黄芪多糖诱导脐血单核细胞向树突状细胞分化中的作用
- Author:
Zhiming CHEN
;
Min DENG
;
Shibing ZHU
;
Xiangqian SUI
;
Xiaoling LI
;
Xiaojie ZHANG
;
Junyang XU
;
Xiaobing DOU
;
Yiqian SHI
- Publication Type:Journal Article
- Keywords:
Cord blood;
Dendritic cells(DCs);
Astragalus polysaccharides(APS)/pharmacology;
Signal transduction pathway
- From:
Chinese Journal of Microbiology and Immunology
2011;31(4):305-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigatethe role of NF-κB played in the process of the cord blood monocytes differentiating into dendritic cells(DCs)induced by astragalus polysaccharide(APS)and to explore the signal transduction pathway involved in this process.Methods Umbilica]cord blood was collected in aseptic conditions.The cord blood monocytes were obtained by density gradient centrifugation and were divided into three groups afterwards.In the control group.cells were cultivated in the RPMI 1640 complete medium.In the APS group.cells were cultivated in the RPMI 1640 complete medium containing 100 mg/L APS.In the PDTC group:cells were treated with 10 μmol/L disulfide carbamate(PDTC).NF-κB inhibitor in 30 min followed by cultivalion in the RPMI 1640 complete medium containing 100 mg/L APS.,The morphological changes were observed during the process of cultivation by the optical microscope and transmission electron microscopy.Cells were collected 12 d later and the cellular immunophenotyping was assayed by FCM.,The activation and migration of NF-κB fluorescence in the cells was examined by the immunoflouresce microscopy.Results (1)Cells in the control group grown up without cluster forformation and were found fusiform and macrophage-like in 12 d.Cells in the APS group grown up in clnstem,and morphological changes were found from the circular shape to a typical dendritic cells-like shape.Cells in the inhibitor group grown up slowly and without cluster formation,and cell morphdogy had no significant change.(2)The expression of DCs-specific antigen CD80,CD83 and CD86 in the APS group was higher than that in the control group and inhibitom group(P<0.01).The expression of those antigen in the control group and PDTC group was similar and had no statistically significance(P>0.05).(3)NF-κB fluorescence in the nuclei was examined by the immunoflourescence microscopy and was much higher in the APS group than that in khe other groups,especially in 72 h with the activation rate of NF-κB (75.20±7.37)%,while(13.20±3.46)% of PDTC group and(8.20 ±1.92)%,respectively(P<0.01).Conclusion Astragalus polysaccharide can induce the differentiation of umbilical cord blood cells into DCs,and NF-κB is the key component of the signal transduction pathway involved in this process.
