Study on the evolution of the minor resistant mutations and the primary resistance in rural areas of Henan
10.3760/cma.j.issn.0254-5101.2011.04.017
- VernacularTitle:河南某农村艾滋病患者劣势耐药毒株的进化及原发耐药研究
- Author:
Hanping LI
;
Wei GUO
;
Xinpeng ZHU
;
Zhe WANG
;
Yongjian LIU
;
Zuoyi BAO
;
Lin LI
;
Daomin ZHUANG
;
Siyang LIU
;
Zheng WANG
;
Xiaolin WANG
;
Jingyun LI
- Publication Type:Journal Article
- Keywords:
Antiretroviral therapy;
Primary drug-resistance;
In-house;
ASPCR
- From:
Chinese Journal of Microbiology and Immunology
2011;31(4):356-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the antiretroviral therapy(ART),analyze the prevalence of resistance in rural areas,Henan,and explore the presence of minor resistant variants in pre-ART.Methods One hundred and forty-nine AIDS patients initiating ART were recruited and investigated at intervals of 6 months. Method of In-house developed by our laboratory for genotypjc resistance test was to analyze the occurrence of resistance among the failure of ART,and the allele-specific real.time PCR(ASPCR)was used to detect the minor resistant variants at the baseline samples once the resistance occurred.Results Vimlload significantly decreased among the patients who received ART(t=275,P=0.0001),but the absolute counts of CD4+T lymphocytes had no significant change(t=1.765 168,P=0.0852).Rate of resistance among the patients of treatment failure was 4.88%.The result of ASPCR in the survey of baseline showed that the minor resistant variants of M184V were detected in 7 patients and mutation K103N presented in 5 patients.Conclusion The primary drug-resistant straias in the untreated patients were found in Henan,and they might develop the dominant resistance strains and bring about the failure of ART.
