Intestinal permeability and injury change in chronic renal failure rats
10.3760/cma.j.issn.1001-7097.2011.05.016
- VernacularTitle:慢性肾衰竭大鼠肠道通透性及损伤的研究
- Author:
Fangfei LI
;
Zongpei JIANG
;
Wenxue HU
;
Yu ZHANG
;
Yafang LI
;
Wenxing PENG
;
Xuchu HU
;
Xueqing YU
- Publication Type:Journal Article
- Keywords:
Gastrointestinal tract;
Permeability;
Kidney failure,chronic;
Rats;
Chromatography,high pressure liquid
- From:
Chinese Journal of Nephrology
2011;27(5):369-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the histopathologic injury of small intestine and intestinal permeability in chronic renal failure (CRF) rats. Methods Twenty male Sprague-Dawley rats were randomly assigned to CRF group (n=10) and control group (n=10). 5/6 nephrectomy was used to establish CRF rats, while sham operation for control. Blood biochemistry was regularly monitored until CRF model was successfully established. The model rats were fed with lactulose (L) and mannitol (M) through intragastric administration. Urine was collected after 6 hours, and the concentration of lactulose and mannitol in urine was measured using high pressure liquid chromatograph with refractive index detector (HPLC-RID), and the ratio of urinary excretion of L/M was calculated to evaluate intestinal permeability. Small intestinal mucosa were stained by hematoxylin-eosin (HE) and observed with light microscope (villus height, thickness of muscle layer and villus count), histological damage score was used to evaluate intestinal injury. Results The L/M ratio of CRF group was higher than that of control group (1.75±0.11 vs 1.20±0.06, P<0.01). The small intestinal mucosal villus height and thickness of muscle layer in CRF group were higher (P<0.01), and the number of villi was lower compared to control group (P<0.01). The score of histopathologic intestine damage of CRF group was higher than that of control group (1.00±0.71 vs 0, P<0.01). Conclusion The intestinal permeability of CRF rats is increased with varying degrees of intestinal damage.
