Quantitative detection of prostate specific membrane antigen and the splicing variant DNA in different tumor cell strains and prostate tissues

VernacularTitle:前列腺特异膜抗原及其变异体DNA在肿瘤细胞株及前列腺组织中定量检测
Author: Haiyan YAN ; Rui CHEN ; Rihui ZHONG ; Xiaohong LUO ; Weisi LAO ; Kaiyuan CAO
Publication Type:Journal Article
Keywords: Reverse transcriptase polymerase chain reaction; Prostate-specific antigen; Protein isoforms
From: Chinese Journal of Geriatrics 2011;30(7):602-604
CountryChina
Language:Chinese
Abstract: Objective To understand and illuminate the bionomic characteristics of prostate specific membrane antigen (PSMA) and splicing variant PSMA5, through detecting the DNA levels of them in different tumor cell strains and prostate tissues. Methods The fluorescent quantization reverse transcriptase PCR (FQ-RT-PCR) method built up by our research group was used to detect the PSMA and variant PSMA5 DNA levels in different tumor cell strains and prostate tissues. Results The PSMA and PSMA5 DNA levels in tumor cell strains and pathological prostatic tissues were obviously more than those of the normal prostatic tissues (F=3.40, 11.94, both P＜0.05), and the PSMA5 DNA level was much higher than was the PSMA DNA level in prostatic carcinoma tissues (P＜0.05). Conclusions The different expressions between PSMA and PSMA5 in different tumor cells and prostatic tissues show that PSMA5 is more specific than PSMA as a prostate carcinoma tumor marker.