The role of TLR2, TLR4 and its signal pathway in gouty arthritis
10.3760/cma.j.issn.1007-7480.2011.05.004
- VernacularTitle:Toll样受体2和Toll样受体4及其信号通路在原发性痛风性关节炎发病机制中作用的研究
- Author:
Li JIANG
;
Jingguo ZHOU
;
Yufeng QING
;
Wenguang XIE
;
Qibin YANG
;
Mingcai ZHAO
;
Yan XING
;
Jin WEI
;
Guohua YUAN
;
Hong JIANG
- Publication Type:Journal Article
- Keywords:
Arthritis,gout;
NF-kappa B;
Interleukin-1;
Toll like receptors 2;
Toll like receptors 4
- From:
Chinese Journal of Rheumatology
2011;15(5):300-304
- CountryChina
- Language:Chinese
-
Abstract:
objective The roles of TLRs and their signal pathway in gouty arthritis(GA)were explored.Methods TLR2 and TLR4 mRNA was measured using real-time quantitative polymerase chain reaction(RT-PCR)in PBMCs,IL-1β level was detected using ELISA in plasma,and NF-κB p65 protein level in PBMCs was measured using Western blot.Level of TLR2 mRNA,ILR4 mRNA,IL-1β,NF-κB p65protein was compared among acute GA,non-acute GA and healthy controls.Correlation between TLR2mRNA,TLR4 mRNA and serum uric acid,IL-1β level in GA patients was analyzed.One-way ANOVA was used to analyze data between multiple groups and q-test was used for two-two comparison.Spearman's analysis was applied for correlation analysis.Resuits The expression of TLR4 mRNA,NF-KB p65 protein,IL-1β arid serum uric acid level in patients with acute GA [(5.0±1.2), (7.11±0.18), (283±83)pg/ml,[585±123)μmol/L] was significantly increased compared to non-acute GA[(2.3±0.4),(0.63±0.06),(134±29)pg/ml,(493±107)μmol/Lj and healthy controls(1.1±0.6),(0.52±0.12),(97±17)pg/ml,(326±65)μmol/L](P<0.01,respectively).Significant diffefence was also observed between non-acute GA patients and healthy controls(P<0.05,respectively).The level of TLRR4 mRNA was positively correlated with uric acid and IL-1β level in GA patients(rs=0.876,0.779;P<0.05,respectively).Conclusion Innate immunity are activated by membrane-type pattern recognition receptors in primary GA.TLR4-NFκB p65-IL-1β signat transduction may participate in the inflammatory mechanisms of gout.Urate crystals in patients with gout may:be involved in the activation of TLR4 and its signal pathway.
